Board Thread:Questions and Answers/@comment-11276487-20131125022524/@comment-5313287-20140111062748

Possible "big" villains from Peters Past that are still alive to fight Miles:

Main guesses
 * Electro (If Nick Fury didn't rally kill him)
 * Kraven (Pretty sure he is still alive)
 * Venom (Just fought him so probably not it)
 * Sandman (Same reason as Kraven)
 * Vulture (Same reason as Kraven)
 * Rhino (Beat him already)

Longshots
 * Scorpion (Peter's other clone, which may or may not be alive)
 * Mysterio (Earth-616, Depends if he escapes back into the Main Universe)
 * Chameleons (Guessing here)
 * Mister Sinister (I feel he wasn't an enemy of Peter's but he is doing something...)