User blog comment:MarvelFan2012/Questions and Answers about Spider-Man/@comment-5347501-20120730021111/@comment-5111260-20120730044132

Wait a minute, I accidentally forgot to mention that he did sleep with Mary Jane when they were married, and she became pregnant with his baby. But after she went into labor, a woman named Alison Mongraine (whom was working for Norman Osborn) had stolen the baby from her and Peter.

But after the One More Day storyline in which Peter's marriage was removed by Mephisto, he was single once again. And then at one point when he rekindled his relationship with the Black Cat, they slept together.

Sorry about not mentioning that.