User blog comment:MarvelFan2012/Questions and Answers about Spider-Man/@comment-5091218-20120908013502/@comment-5111260-20120908014604

You mean Madame Web. During a storyline called the Grim Hunt, she was killed by Sasha Kravinoff after she slashed her throat open.

But before she died, she passed on her powers to Julia Carpenter, the second Spider-Woman (before later calling herself Arachne). As of now, Julia is the new Madame Web.