User blog comment:MarvelFan2012/More questions and answers about Spider-Man/@comment-4136718-20130324220203/@comment-5111260-20130324221517

He lost all of his enhanced powers after the events of One More Day in which he made a deal with Mephisto which reset his powers back to the way they were.

As for the use of his stingers, they were rarely ever used by Spider-Man except whenever he was confronted by those associated with his mystical powers, like Morlun, Ezekiel or Miss Arrow. Just like the rest of his increased powers during One More Day, he lost the stingers.