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Anticipating that the Venom symbiote would develop a resistance to the treatment he'd developed for its toxic shock syndrome, Doctor Steven began work on a permanent cure in the form of an astrobiological serum derived from the Anti-Venom symbiote. When Eddie Brock confronted him about the Venom symbiote's growing bloodlust, Dr. Steven showed him the serum, which he warned could potentially kill the symbiote if too much was used. Their argument was interrupted by Flash Thompson, who sought to reclaim the symbiote from Eddie. As the symbiote tried to bond to both of them at the same time, Spider-Man arrived and attempted to kill the alien by overturning one of the vats; though this failed and had the unintended consequences of curing Eddie's metabolic instability and turning the portion of the Venom symbiote bonded to Flash into a new Anti-Venom symbiote.[1]

When Lee Price began forcibly recruiting criminals and supervillains into his own criminal syndicate using his ill-obtained symbiote, Spider-Man, Agent Anti-Venom, Black Cat, and Mania used the astrobiological serum to free those under his control.[2][3]

When Norman Osborn bonded to the Carnage symbiote to become the Red Goblin, Spider-Man thought to use the remaining astrobiological serum against him. However, Osborn beat him to it and destroyed the serum.[4]

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