- Serpent Society
- Constrictor (Frank Payne) (Final appearance)
- Bushmaster (Quincy McIver) (Final appearance)
- Death Adder (Roland Burroughs) (Appears on a computer screen, TV, or hologram only) (Final appearance)
- King Cobra (Klaus Voorhees) (Final appearance)
- Rattler (Gustav Krueger) (Final appearance)
- Anaconda (Blanche Sitznski) (Final appearance)
- Fantastic Four
- Iron Fist (Danny Rand) (Cameo)
- Luke Cage (Cameo)
- Spider-Man (Peter Parker) (Cameo)
- "Mole People" (Mentioned)
Wasp and Iron Man try to persuade Hank Pym to rejoin the Avengers. However, he's far too obsessed with his experiments and driven by guilt over Ultron. Meanwhile - a new vigilante called 'Yellowjacket' emerges.
- At roughly 10 minutes into the episode, buildings in the background include Oscorp and Damage Control.