Appearing in "Now, By My Hand, Shall Die a Villain!"
- Masters of Evil
- Numerous unnamed New Yorkers
- South American Natives
- 🢐 Franz Gruber 🢒 (Unnamed)
- Nazis (Referenced)
- Nick Fury (Name only)
- U.S. Mail (Name only)
- Black Knight's Steed
Races and Species:
- Solar System
- Asgard (Referenced)
- Captain America's Uniform and Shield
- Iron Man Armor MK II
- Giant-Man's Suit
- Wasp's Suit
- Bio-Synthetic Wings
- Black Knight Armor
- Black Knight's Power Lance
- Melting Ray MK I
- XL-750 Hunter Rocket Plane (First and only known appearance)
- World War II (Name only)
Synopsis for "Now, By My Hand, Shall Die a Villain!"
Zemo decides again to attack the Avengers, and so his agents kidnap Rick Jones. Zemo also contacts the Executioner and the Enchantress and they set free the Black Knight and the Melter.
The four villains attack the Avengers while Captain America sets off to the Amazon to rescue Rick. The Avengers and villains in New York fight to a stand still. In the Amazon, Captain America rescues Rick but is then ambushed by Zemo. Captain America is too skilled however, and Zemo accidentally triggers an avalanche which lands on and kills him.
- Giant-Man issues the astiring cry, "Avengers Assemble" in this issue.
- Inker Mike Esposito uses the pseudonym "Mickey Demeo" this issue.
- Thor is last seen in Journey Into Mystery Vol 1 114 where he battled the Absorbing Man.
- Iron Man and Captain America were last seen in Tales of Suspense Vol 1 63, where Iron Man battled the Phantom and Captain America retold his origins.
- Giant Man and Wasp were last seen in Tales to Astonish Vol 1 65 where Giant Man got the new costume shown in this issue of Avengers.
- Baron Zemo was last seen in Tales of Suspense Vol 1 60, where he battled against Captain America with his Army of Assassins. This issue marks Baron Zemo's death, he is succeeded by his son Helmut Zemo who makes his first appearance in Captain America Vol 1 168. Helmut is shown having assumed the mantle of Zemo in Captain America Vol 1 274.
- Enchantress and the Executioner were both last seen in Avengers Vol 1 10 when they encountered Immortus.
- The Melter last seen in Avengers Vol 1 6 during the first formation of the Masters of Evil. He was captured after that battle.
- The Black Knight was last seen in Tales of Suspense Vol 1 59 where he briefly escaped prison and battled Iron Man before being captured and imprisoned once again.
- The plot of the letter that Captain America writes and mails to Nick Fury does not get addressed again until Avengers Vol 1 19.
- Iron Man next appears (chronologically) in Avengers Vol 1 16.
- Cap next appears (chronologically) in Avengers Vol 1 16.
- Giant Man and Wasp next appear (chronologically) in Avengers Vol 1 16.
- Thor appears next (chronologically) in Avengers Vol 1 16, as Journey Into Mystery Vol 1 115 is a continuation of Thor's battle against Absorbing Man from Journey Into Mystery Vol 1 114.