The Brood Queen was a member of The Six Fingered Hand who killed the Punisher's family at request of the Watchers. At some point she and her entire race bonded with there own symbioates. She was the first one he tracked down and while Punisher distracted the Queen, Chip gained intel of where to find the next member and planted a blackout bomb. After teleporting out and flying away, they activated the bomb which killed the Queen and her Brood.[1]

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