Appearing in 1st story
Featured Characters:
- Spider-Man (Peter Parker) (Main story and flashback)
Supporting Characters:
- Scarlet Spider (Kaine Parker) (Main story and flashback)
- Web-Warriors (Main story and flashback)
- Karn (Earth-001)
- Spider-Woman (Gwen Stacy) (Main story and flashback) (Impersonates Gwen Stacy)
- Parker Industries (Main story and flashback)
- The Living Brain (Only in flashback)
- Anna Maria Marconi
- Horizon University
Antagonists:
- Alternate San Franciscans infected by the Carrion Virus
- Max Modell
- Bella Fishbach
- Grady Scraps
- Unidentified others (Dream)
- New U Technologies
- Gwen Stacy
- Doctor Octopus (Otto Octavius) (Main story and flashback) (Possessing The Living Brain in flashback only)
- Jackal ("Miles Warren")
- Miles Warren Clones
- Rhino (Aleksei Sytsevich)
- Electro (Francine Frye)
- Lizard (Dr. Curt Connors)
- Dr. Martha Connors
- Spider-Slayer (Alistaire Smythe) (Returns)
- Jack O'Lantern (Steven Levins)
- Massacre (Marcus Lyman)
- Mirage (Desmond Charne)
- Big Man (Fred Foswell) (Returns)
- Ox (Raymond Bloch) (Returns)
- Montana (Jackson Brice)
- Spencer Smythe (Returns)
- Stilt-Man (Wilbur Day) (Returns)
- Hobgoblin (Jason Macendale) (Returns)
- Tarantula (Anton Miguel Rodriguez)
- Eduardo Lobo (Returns)
- Mysterio (Francis Klum) (Returns)
- Hitman (Burt Kenyon) (Returns)
- Kangaroo (Frank Oliver)
- Rose (Richard Fisk) (Returns)
- Green Goblin (Dr. Barton Hamilton) (Returns)
- Dr. Marla Madison-Jameson
- Spider-Woman (Mattie Franklin) (Returns) (Shadow only)
- George Stacy
- Jean DeWolff (Returns)
- Prowler (Hobie Brown)
- Dr. Ashley Kafka (Returns)
- Unidentified members (Appear in shadow only)
Other Characters:
- Spider-Army (Only in recap)
- Spider-Man (Otto Octavius / "Peter Parker") (Only in recap)
- Spider-Man (Ezekiel Sims) (Only in recap)
- Spider-Girl (Anya Corazon) (Only in recap)
- Spider-Man (Peter Parker) (Only in recap)
- Spider-Man (Miles Morales) (Only in recap)
- Spider-Man (Hobie Brown) (Only in recap)
- Spider-Woman (Ashley Barton) (Only in recap)
- Spider-Monkey (Peter Parker) (Only in recap)
- Spider-UK (Billy Braddock) (Only in recap)
- Spider-Man (Miguel O'Hara) (Only in recap)
- Spider-Ham (Peter Porker) (Only in recap)
- Spider-Woman (Jessica Drew) (Only in recap)
- Silk (Cindy Moon) (Only in recap)
- Anna Maria Marconi (A.I.) (Voice only) (Only in flashback)
- Abby-L (Mentioned)
- Ben Reilly (Mentioned)
- Joyce Delaney (Mentioned)
- Spider-Skeleton (Mentioned)
- Guardian (Mentioned)
- Jack (Mentioned)
- Spidercide (Mentioned)
- Anubis (Mentioned)
- Billy Connors (Mentioned)
- Kingpin (Wilson Fisk) (Mentioned)
- Vanessa Fisk (Mentioned) (Deceased)
- Fact Channel News (Mentioned)
- God (Yahweh) (Invoked)
- Jay Jameson (Mentioned)
- Alternate Parker Industries (Earth-Unknown)
- Alternate Benjamin Reilly (Earth-Unknown)
- Alternate New U Technologies (Named only)
Races and Species:
Locations:
- Unidentified alternate reality (World destroyed by the Carrion Virus - Epicenter: San Francisco)
- Prime Marvel Universe
- Earth (Main story and flashback)
- United States of America (Main story and flashback)
- Hell (Invoked)
- Earth (Main story and flashback)
- Earth-001 (Only in recap)
- Loomworld (Only in recap)
- Earth-65 (Mentioned)
Items:
- Carrion Virus
- Spider-Men's Suits (Main story and flashback)
- Scarlet Spider's Suit (Main story and flashback)
- Spider-Armor MK IV
- Superior Spider-Man's Suit (Only in flashback)
- Traditional Red and Blue Suit (Only in flashback)
- Ultimate Spider-Man's Suit (Only in flashback)
- Spider-Man 2099's Suit (Only in flashback)
- Dimensional Travel Watch (Dream)
- Web-Shooters (Main story and flashback)
- New U Pills (Mentioned)
- Doctor Octopus' Tentacles
- Spider-Women's Suits (Only in flashback)
- Jackal's Helmet
- Rhino Armor
- Spider-Slayer Mark I
- Stilt-Man's Suit
- Goblin Armor
- Prowler's Suit
Vehicles:
- Jack O'Lantern's Platform
- Goblin Glider
- Spider-Mobile (Mentioned)
Synopsis for 1st story
- Synopsis not yet written
Solicit Synopsis
• The surprises keep coming in the SPIDER-MAN EVENT OF THE YEAR!
• Doc Ock is back and it’s time for him and the Amazing Spider-Man to throw down!
• What lurks in the Underground?!Trivia
- At one point, Jackal references Yoda.