Appearing in "Guest Starring: Hell Cow"
- Doctor Kilgore (First and only known appearance; dies) (Main story and flashback)
- Dracula (Only in recap)
- Hans (Only in recap)
- Alexander (Mentioned)
- Axel Alonso (Mentioned)
- Sebastian Girner (Mentioned)
- Hellcow (Bessie) (Death)
- 50 alternate Deadpools
Races and Species:
- Humans (Main story and flashback)
- Cows (Main story and flashback)
- Duckworldians (Only in flashback)
- Vampires (Only in recap)
- Triceratops (Dream)
- Earth (Main story and recap)
- Heaven (Mentioned)
- Hell (Invoked)
- Unidentified reality (Hellcow died in the Sun)
- 50 other equivalent realities (Hellcow died in the Sun)
Synopsis for "Guest Starring: Hell Cow"
- Synopsis not yet written
Watch out Marvel U, the Bovine Blood Beast is back!" The Merc with a Mouth meets the "The Cow with a Cape. Gaze in horror, dear reader, upon the fiendish experiments inflicted upon Deadpool and Bessie the Vampire Cow by the malevolent Dr. Kilgore in the name of mad science and bear witness to their terrible revenge! Nothing on earth can prepare you for the horror that lurks within these pages.
- The flashbacks are of issue Giant-Size Man-Thing #5