Appearing in "Bullseye Part One: The Shaft"
- Dark Avengers
- Judith (First appearance) (news anchor)
- Gavin (First and only known appearance; dies)
- Skrulls (Appears on screen)
- H.A.M.M.E.R. (Mentioned)
- Thunderbolts (Mentioned)
- Hawkeye (Clint Barton) (Mentioned)
- Tanya Patterson (Mentioned)
Races and Species:
Synopsis for "Bullseye Part One: The Shaft"
Deadpool is still after Norman Osborn for his money, so Norman sends someone with some personal history with ol' Wade - Bullseye. Deadpool is hiring himself out on Craigslist and kills a guy for giving a girl back in school a reputation that has ruined her life, and is now robbing the guy whose house he lured him to. But Bullseye kills the guy and then him and Wade go at it, with Deadpool getting an arrow through his head.
- The pizza scene involving Gavin was adapted into the Deadpool movie with a few modifications, most notably with Gavin's stand-in, Jeremy Garrett, being let go unharmed.