- Adrian Pasdar as Iron Man
- Roger Craig Smith as Captain America
- Travis Willingham as Thor Odinson
- Fred Tatasciore as Hulk
- Troy Baker as Hawkeye, Loki
- Laura Bailey as Black Widow, Sif
- Samuel L. Jackson as Nick Fury
- Colleen O'Shaughnessey as Wasp
- Jennifer Hale as Captain Marvel
- David Kaye as J.A.R.V.I.S., Mysterio
- Bumper Robinson as Falcon
- Charlie Adler as M.O.D.O.K.
- Drake Bell as Spider-Man
- Logan Miller as Nova
- Greg Cipes as Iron Fist
- Matt Lanter as Venom
- Courtnee Draper as White Tiger
- Ogie Banks as Power Man
- Tara Strong as Black Cat
- Nolan North as Green Goblin, Rocket Raccoon
- Tom Kenny as Doctor Octopus
- J. K. Simmons as J. Jonah Jameson
- Chris Cox as Star-Lord
- Nika Futterman as Gamora
- Kevin Michael Richardson as Groot
- David Sobolov as Drax
- Jeff Bennett as The Collector
- Carlos Alazarqui as Cosmo the Spacedog
- Chris Edgerly as Yondu Udonta
- James C. Mathis III as Ronan the Accuser
- Ryan Potter as Hiro Hamada
- Scott Adsit as Baymax
