- Elena "Yo-Yo" Rodriguez (Main story and flashback)
- S.H.I.E.L.D. (Main story and flashback)
- Francisco Rodriguez (Mentioned) (Deceased)
Races and Species:
- Earth-199999 (Main story and flashback)
- Quinjet (Only in flashback)
Colonel Ramon tries to taunt Yo-Yo into shooting him, but she realizes doing so would be wrong. Just as she lowers her gun, a Watchdog appears behind her with the Hydra weapon and fires it. Yo-Yo uses her powers to snatch the weapon out of the Watchdog's hands, but the beam hits Colonel Ramon, killing him instantly. Daisy knocks him out, and she and Yo-Yo leave the warehouse before S.H.I.E.L.D. can swarm the area. In the present day, Daisy wipes the record of Yo-Yo going to Baltimore from her S.H.I.E.L.D. watch. Yo-Yo takes out the S.H.I.E.L.D. medal Coulson gave her months ago and gives it to Daisy.
|Elena "Yo-Yo" Rodriguez||Natalia Cordova-Buckley|
|Quake / Agent Daisy Johnson||Chloe Bennet|
|Col. Victor Ramon||Yancey Arias|
|Alpha Dog||Dale Pavinski|
|Henchman #1||Matt Berberi|
|Henchman #2||Hiroo Minami|
- Dale Pavinski, who portrays the main Watchdog, also portrayed the Watchdog Briggs in the Marvel's Agents of S.H.I.E.L.D. episode "Uprising." It is unknown if these two characters are supposed to be one in the same.