- Rhino (Alex O'Hirn)
- Lizard (Curt Connors)
- Doctor Octopus (Otto Octavius)
- Electro (Max Dillon)
- Green Goblin (Norman Osborn)
- Kangaroo (Brian Hibbs) (Mentioned)
Take on the role of Spider-Man and use your Web Shooters to help defeat five of his all-time greatest rivals! Travel the rooftops of New York City and battle against The Rhino, The Lizard, Electro, Doctor Ock’s Octobots, and The Green Goblin!