- Spider-Man (Peter Parker) (Briefly bonds with a synthetic symbiote)
- Max Modell
- Miles Morales
- Gwen Stacy
- Anya Corazon
- Venom (Curt Connors) (Briefly bonds with Venom) (First appearance as Venom)
- Grady Scraps (Joins Horizon High)
- Aunt May
- Venom (V-252) (Apparent death) (Assimilates a synthetic symbiote)
- Technovore (First appearance)
- Vulture (Adrian Toomes) (Appears on a computer screen, TV, or hologram only)
- Electro (Appears on a computer screen, TV, or hologram only)
- Sandman (Flint Marko) (Appears on a computer screen, TV, or hologram only)
- Iron Man (Tony Stark) (Mentioned)
- Captain America (Mentioned)
- Hulk (Mentioned)
- Vision (Mentioned)
- Anna Watson (Mentioned)
- Mary Jane Watson (Mentioned)
- Harry Osborn (Mentioned)
Despite being incapacitated following his latest battle with Spider-Man, Venom sends a piece of himself to unleash a weapon of unknown purpose around the same time Peter and Max prepare to study the mutant symbiote.
- Max creates an artificial replica of the V-252 symbiote that doesn't have a will of its own.
- It is revealed that Max found out about Spider-man's true identity during the Goblin nation arc.
- The origin of Max's fascination with the V-252 originates from an accident that took place years ago.