Pyko is one of several animal test subjects evolved by a collection of robots. Pyko later led a rebellion with other test subjects against their robot overlords. Using technology taken from their enemies, Pyko evolved three of his soldiers - Ranger, Blackjack, and Wal Rus - into even more dangerous weapons to fight the robots. However, Ranger's younger brother, Rocket, de-evolved all of them back to their original state. Pyko swore his revenge as he reverted into a normal turtle.
- Brian George voices Pyko in the Marvel's Guardians of the Galaxy (animated series) episode "We Are Family."