Appearing in "Big Trouble"
- Spider-Boy (Pete Ross) (Main story and flashback) (First full appearance)
- Betty Brant
- Challengers of the Fantastic
- Insect Queen (Mary Jane Watson)
- Daily Bugle
- Project Cadmus
- Rex Leech
- Special Crimes Unit (First appearance)
- Bruce Wayne (Mentioned)
- Peter Parker (Only in flashback) (Only appearance; dies)
- Thunderbolt Ross (Only in flashback) (Only appearance; dies)
Races and Species:
- Spider-Boy's Gun (First appearance)
Synopsis for "Big Trouble"
- Synopsis not yet written.
- First and only known appearance to date besides flashbacks
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