If I'm going to be serious from now on, I'm going to need a new gimmick, like a gnarly eyepatch, or a fake limp, or a talking Chia Pet. 
Appearing in "No Laughing Matter"
Featured Characters:
Supporting Characters:
- Valeria Colon (First appearance)
Antagonists:
- Slapstick (Steve Harmon)
- El Tenor's henchmen (First appearance)
- El Tenor (Ferraro Colon) (First appearance)
Other Characters:
- May Parker-Jameson (Behind the scenes)
- Daily Bugle (Named only)
- Vulture (Adrian Toomes) (Photo)
- Daily Bugle (Mentioned)
- Ben Parker (Mentioned) (Deceased)
- Gwen Stacy (Mentioned) (Deceased)
- J. Jonah Jameson (Mentioned)
- NYPD (Cameo)
- Ellie Camacho (Mentioned)
- Doop (Vision or hallucination)
- Spider-Ham (Peter Porker) (Vision or hallucination)
- Deadpool the Duck (Vision or hallucination)
- Rocket Raccoon (Vision or hallucination)
Races and Species:
- Humans
- Pig-Spider Hybrids (Vision or hallucination)
- Duckworldians (Vision or hallucination)
- Halfworlders (Vision or hallucination)
Locations:
- Earth
- United States of America
- New York
- New York City
- Manhattan
- Midtown
- Baxter Building (Mentioned)
- Empire State Building (Cameo)
- Washington Heights (Cameo)
- Midtown
- Manhattan
- New York City
- George Washington Bridge
- New Jersey
- Hudson County
- Hoboken
- Daily Bugle Archives (First appearance)
- Elysian Park (First appearance)
- A Taste Cuba (First appearance)
- Hoboken
- Harmon Residence (67 Hazelwood, Plainfield, Union County) (Mentioned)
- Hudson County
- New York
- Cuba (Named only)
- United States of America
Items:
- Spider-Man's Suit (Photo)
- Vulture's Wings (Photo)
- Spider-Armor MK IV
- Web-Shooters
- Deadpool's Suit and Katanas
Gertie (Slapstick's mallet)
Synopsis for "No Laughing Matter"
- Synopsis not yet written
Solicit Synopsis
• The adventures of your favorite duo in comic books continue, and this time Spider-Man and Deadpool have decided NOT TO BE FUNNY ANYMORE?!
• What could have possibly driven our two heroes to take the funny outta their own funny book?
• And just what the heck has SLAPSTICK got to do with it?!