- Felicia Hardy (First appearance as the Black Cat)
- Daily Bugle
- John Hardesky/The Cat (Final appearance)
- Anastasia Hardy
- Glory Grant (Mentioned)
- Blade (Mentioned)
- Daredevil (Cameo) (Appears on a computer screen, TV, or hologram only)
- Kingpin's stealth sky ship
Felicia Hardy has been transformed in the Black Cat by the Kingpin, who has successfully had the Super-Soldier Serum recreated after obtaining the formula from John Hardesky. To test the formula's effects, Felicia is forced to rob a jewelry store. There, she encounters Spider-Man, who she defeats and kisses, although he is unaware of her identity. After returning to Crime Central, Felicia is taken to a holding cell by the Kingpin's men along with her father.
The following day, J.J. Jameson tells Peter that Anastasia Hardy is staying at his penthouse, since she is afraid to return home. Spider-Man decides to visit Felicia's mother, and gives her a Spider-Tracer to use to contact him.
In their cell, Hardesky decides to help his daughter escape. The Black Cat crawls through the ventilation system and out of the fortress. Felicia then returns home to her mother, who gives her Spider-Man's Spider-Tracer. Black Cat uses it to meet with Spider-Man, but they are ambushed by S.H.I.E.L.D. agents, who chase them, wanting to unmask Spider-Man. The two escape on a motorcycle, and Black Cat suggests that they save John Hardesky.
The Black Cat returns to the Kingpin's fortress, without the crime lord ever knowing that she'd left. The Kingpin then orders that Hardesky and Felicia be executed, and tells Landon to manufacture more Super-Soldier Serum. However, Hardesky infiltrates his lab and erases the formula. At the same time, the Kingpin's men prepare to execute Black Cat and Spider-Man, who is disguised as the Cat. They attempt to escape, but Dr. Octopus appears and restrains Black Cat. S.H.I.E.L.D. agents suddenly infiltrate the ship, causing it to explode, although everyone aboard manages to escape safely.
After, Felicia and Anastasia say goodbye to John, he is then taken back into S.H.I.E.L.D. custody.