Appearances
Featured Characters:
- Spider-Man (Peter Parker) (Last appearance)
- Venom (Eddie Brock (Last appearance)
Antagonists:
- The Jury (First appearance)
- Sentry (Curtis Elkins) (First appearance)
- Ramshot (Samuel Caulkin) (First appearance)
- Screech (Maxwell Taylor) (First appearance)
- Bomblast (First appearance)
- Firearm (First appearance)
- Wysper (Jennifer Stewart} (First appearance)
- Life Foundation Symbiotes (First appearance)
- Riot (Trevor Cole) (First appearance)
- Phage (Carl Mach) (First appearance)
- Lasher (Ramon Hernandez) (First appearance)
- Agony (Leslie Gesneria) (First appearance)
- Scream (Donna Diego) (First appearance)
- Carnage (Cletus Kasady) (Final surprise boss) (Last appearance)
Other Characters:
Some other heroes and anti-heroes aid Venom and Spidey throughout the game, making brief cameos to attack the player's foes.
- Captain America (Steve Rogers) (Last appearance)
- Ghost Rider (Johnny Blaze) (First appearance)
- Hawkeye (Clint Barton) (First appearance)
- Daredevil (Matt Murdock) (First appearance)
Locations:
- Earth-33734 (Last appearance)
Items:
- Spider-Man's Suit and Web-Shooters (Last appearance)
- The Venom Symbiote (Last appearance)
- The Carnage Symbiote (Last appearance)
- The Riot Symbiote (First appearance)
- The Phage Symbiote (First appearance)
- The Lasher Symbiote (First appearance)
- The Agony Symbiote (First appearance)
- The Scream Symbiote (First appearance)
- Captain America's Uniform and Shield (Last appearance)
- Ghost Rider's Chain
- Hawkeye's Bow and Trick Arrows
- Daredevil's Billy Club
Vehicles:
- Digger (First appearance)
Synopsis
Spider-Man and Venom team up against the new threat of the Life Foundation, and their five newly spawned symbiotes: Having escaped from the clutches of the treacherous Life Foundation, Venom seeks the aid of his most hated foe, Spider-Man! A tenuous alliance is formed as the Lethal Protector and the Amazing Web-Slinger race to stop the life Foundation from using the symbiotes spawned by Venom in their evil undertakings.
The player/players travel through 18 levels until the Life Foundation's plans are utterly foiled.
- City Streets
- The Bridge
- The Woods
- Enter The Foundation
- Underground City
- Mall
- Vents
- Internal Life Foundation
- Security Zone 1
- Hi-Tech Green House
- Warehouse
- Security Zone 2
- Carnage Resting Place
- Trap 1
- Trap 2
- Trap 3
- Trap 4
- Trap 5
Notes
- Despite the title of Venom: Separation Anxiety, the game mostly consists of the story comprising Venom's Venom: Lethal Protector mini-series.