- —Minka Tross
Appearing in "Bloodline: Part IV"
- Ironheart (Riri Williams) (First appearance as Ironheart)
- The Marker (Faye Ito)
- Tony Stark
- Peter Parker (Main story and flashback)
- Minka Tross (First appearance)
- Cadaverous (Ivan Renz) (Main story and flashback) (Real name first revealed)
- Cadaverous' children
- Hulk (Bruce Banner)
- Thor (Odinson)
- Captain America (Steve Rogers)
- Black Widow (Natasha Romanoff)
- God (Yahweh) (Invoked)
- Stark Industries (Only in flashback)
- Howard Stark (First appearance; flashback only)
- S.H.I.E.L.D. (Mentioned)
- Mary Parker (First appearance; flashback only)
- Richard Parker (First appearance; flashback only)
- Radioactive Spider (First appearance; flashback only)
- Mary Jane Watson (Only in flashback)
- May Parker
Races and Species:
- Hell (Invoked)
- Spider-Man's Suit and Web-Shooters
- Mjolnir (Statue)
- Captain America's Uniform and Shield
- Ironheart Armor Model 3 (First appearance)
Synopsis for "Bloodline: Part IV"
- Synopsis not yet written
• Peter Parker is in the hands of Cadaverous and only his son Ben can save him.
• You may think you know where this story is going, but there are so many surprises coming!