Appearing in 1st story
Featured Characters:
- Spider-Man (Miles Morales) (Main story and flashback)
Supporting Characters:
- The Spinster (First appearance) (Main story and flashback)
- Spider-Gang (Main story and flashback)
- Spider-Monster (Main story and flashback)
- Spider-Zero (Main story and flashback)
- Sea-Spider (First appearance) (Main story and flashback)
- Spider-Ma'am (May Parker) (Main story and flashback)
- Spider-Requiem (Poly Swan) (First appearance) (Main story and flashback)
- Garden-Spider (Petunia Parker) (First appearance) (Main story and flashback)
The Spider-Man (Peter Parker) 
(Main story and flashback)- Spider-Sting (First appearance) (Main story and flashback)
- V (First appearance) (Main story and flashback)
- Web-Slinger (Patrick O'Hara) (Main story and flashback)
- Spider-Horse (Widow) (Main story and flashback)
- Sun-Spider (First appearance) (Main story and flashback)
- Spidair (First appearance) (Main story and flashback)
- White Widow (Venice Doadi) (First appearance) (Main story and flashback)
- Lord Spider (Main story and flashback)
- Sp//dr (Peni Parker) (Main story and flashback)
- Spider-Man (Takuya Yamashiro)
- Spiderling / The Patternmaker (Annie Parker)
Antagonists:
- Machine Bem Monsters (First appearance)
- Genyouchu
- Samson
- Mer-Man
- Cat Demon Monster
- Snake Woman
- Sasora
- Biker Monster
- Kamenger
- Monkfish
- Primitive Man
- Centipede
Other Characters:
- Mary Janes (Named only)
- Great Weaver (Mentioned)
- Iron Cross Army (Mentioned)
Races and Species:
- Humans (Main story and flashback)
- Horses (Main story and flashback)
- Spiders (Main story and flashback)
- Rats (Mentioned)
- Gods (Mentioned)
- Demons (Mentioned)
Locations:
- Earth-001
- Earth-51778 (Behind the scenes)
- Hell (Mentioned)
- Spider-Zero's reality (Only appearance; destruction in flashback)
- Earth (Only appearance; destruction in flashback)
- Prime Marvel Universe
Items:
- Web of Life and Destiny
- Spider-Men's Suits (Main story and flashback)
- Spider-Ma'am's Suit (Main story and flashback)
- Spider-Man's Noir Suit (Main story and flashback)
- SP//dr Suit (Main story and flashback)
- Spider Idol
- Web-Shooters (Main story and flashback)
- Spider Bracelet
- Spiderling's Suit
Vehicles:
Events:
- Incursion (Only in flashback)
Synopsis for 1st story
Spider-Zero using the idol discovers that Annie finds herself trapped in a tangle that has formed in the web. The tangle is sentient and feels nothing but pain and an instinctive need to protect Annie. And always because of the tangle if the canvas is sick. Spider-Zero gathers an army of spiders, but and the tangle counterattacks with one of monsters, but eventually Spider-Zero manages to untie it and is then saved by Miles, who can finally go home.
Solicit Synopsis
• The conclusion to SPIDER-VERSE is here!
• All the various threads of this series (Miles Morales, Spider-Man Noir, SP//dr, Webslinger, Spider-Ma’am, and all the other Spider-People and Spider-Sonas) come together to re-spin the Web of Life and Destiny!
• But the journey won’t be easy, so don’t be surprised if all the spiders don’t make it through alive!
