- Spider-Man (First appearance)
- Doctor Octopus (Only appearance)
- Hobgoblin (First appearance)
- Mysterio (First appearance)
- Rhino (Only appearance)
- Scorpion (First appearance)
- Venom (Only appearance)
Races and Species:
- Spider-Man's Suit
- Doctor Octopus' Tentacles
- Goblin Armor
- Hobgoblin Glider
- Pumpkin Bombs
- Mysterio Suit
- Rhino Armor
- Scorpion's Suit
Spider-Man's most dangerous super villains have discovered his secret identity, Peter Parker, and kidnapped his wife, Mary Jane. The action game takes the player through various city locations, battling an assortment of minor thugs, animals, and a supervillain at the end of each level who, through intermission scenes, will taunt Spider-Man as to the whereabouts of his wife.
- This is the first appearance of Venom in a video game.
- In the game manual, the screencap accompanying the Hobgoblin description depicts the Green Goblin instead. However, the game correctly has Hobgoblin. It's possible that the Green Goblin was included at an early version before changes were made, as within the game, Spider-Man calls Hobgoblin "Gobby" as opposed to "Hobby"
- Spider-Man never refers to his enemies by their full name in this game; only the manual shows their names.
This title was followed by two sequels, The Amazing Spider-Man 2 and The Amazing Spider-Man 3: Invasion of the Spider-Slayers.