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- Spider-Man (Peter Parker) (Main story and flashback)
- Hulk (Bruce Banner)
- S.H.I.E.L.D. Trainees
- Nova (Sam Alexander) (Cameo)
- Iron Fist (Danny Rand) (Cameo)
- White Tiger (Ava Ayala) (Cameo)
- Power Man (Luke Cage) (Cameo)
- New Warriors
- Genetically-modified Spider (Only in flashback)
- This episode aired in the UK on October 18, 2014.