Peter Parker has been Spider-Man for a year. He has saved lives and fought supervillains, but is still in the process of learning how to be a superhero. Nick Fury of S.H.I.E.L.D. offers Peter the chance to train to be a real superhero and become "The Ultimate Spider-Man". However, Peter will first have to learn how to work with a team of four fellow teenage superheroes who are also under the training program of S.H.I.E.L.D. .The teen heroes that Spider-Man will be teaming up with are Nova, White Tiger, Iron Fist, and Power Man.
Following the fight with the Green Goblin which resulted in the destruction of the S.H.I.E.L.D. Helicarrier, Spider-Man's team ends up staying at Peter Parker's house. The first four episodes of the season feature the first appearances in this series of Lizard, Electro, Rhino, and Kraven the Hunter. Episode 5 features a team-up with the Avenger Hawkeye and Episode 6 the first appearance of the Sinister Six lead by Doctor Octopus.
Season Three: Web-Warriors
Disney XD and Marvel officially announced Season Three on July 20, 2013 at San Diego Comic Con. It will be renamed "Ultimate Spider-Man: Web-Warriors". Season Three will include Spider-Man joining The Avengers, and introducing characters such as Cloak and Dagger, Amadeus Cho, Squirrel Girl, Ka-Zar, Scarlet Spider, and Agent Venom. Miles Morales, Spider-Girl, Spider-Man Noir, and Spider-Man 2099 will also make appearances as well.
Season Four: Ultimate Spider-Man vs The Sinister 6
In season four, the animation was given a name change again to "Marvel's Ultimate Spider-Man vs. The Sinister 6." The season starts out with a confident Spider-Man who feels like the ultimate hero after fighting alongside The Avengers and leading a team of heroes at S.H.I.E.L.D. Academy. However, when the wall-crawler discovers that his greatest enemies – Doctor Octopus, Hydra and the all-new Sinister 6 – are working together to destroy him and S.H.I.E.L.D., he teams up with a special new team of Spiders including Miles Morales, Agent Venom, Iron Spider and the mysterious new Scarlet Spider. Spider-Man and his new friends will need to use all of their power to overcome their greatest challenge yet.
In October 2016, Marvel announced that Ultimate Spider-Man would come to an end in the following January with the two-part final arc "Graduation Day."