Appearances
Featured Characters:
- Spider-Man (Peter Parker) (Main story and flashback)
Supporting Characters:
- S.H.I.E.L.D.
- Web-Warriors
- Agent Venom (Flash Thompson) (Main story and flashback)
- Stan
- Patrioteer (Harry Osborn) (Main story and flashback) (Joins S.H.I.E.L.D. and New Warriors)
- Web-Warriors
Antagonists:
- Carnage (Symbiote) (Main story and flashback)
- Carnage Queen (Mary Jane Watson) (First appearance as Carnage Queen) (Bonds to and loses the Carnage Symbiote)
- Hydra
- Doctor Morbius (Apparent death)
- Crossbones (Brock Rumlow)
Other Characters:
- S.H.I.E.L.D. Trainees
- Doctor Octopus (Otto Octavius) (Mentioned)
- Aunt May (Peter Parker's imagination only)
Locations:
Items:
- Spider-Man's Suit
- Spider-Man's Web-Shooters
- Venom Symbiote (Main story and flashback)
- Carnage Symbiote
- Anti-Venom Symbiote (Only in flashback)