Appearing in 1st story
- Tomas (First appearance)
- Mew (First appearance)
- Spider-Man (Peter Parker) (Mentioned)
- Giganto (Photo) (Cameo)
Races and Species:
- Squirrel Girl's Costume (First appearance)
- Deadpool's Guide to Super Villains Cards (First appearance)
Synopsis for 1st story
- Synopsis not yet written
• Wolverine, Deadpool, Doctor Doom, Thanos: There’s one hero that’s beaten them all — and now she’s got her own ongoing series! (Not that she’s bragging.)
• That’s right, you asked for it, you got it, it’s SQUIRREL GIRL! (She’s also starting college this semester.)
• It’s the start of a brand-new series of adventures starring the nuttiest and most upbeat super hero in the world!
- The series' logo was designed by Mike Allred.
- Maris Wicks is credited for the art in the trading cards from Deadpool's Guide to Super Villains.
- Almost every page of this issue contains a small text at the bottom of it, from the point of view of the writer.
- In this issue, Kraven mentions his curse to be unable to die unless at the hands of Spider-Man. However, he was lifted of his curse in the pages of Scarlet Spider.
- On December 24, 2014, writer Ryan North published a writer's commentary on this issue online. These notes were later printed in the letters section of Unbeatable Squirrel Girl (Vol. 2) #42 in 2019.