Important LGBTQ characters:
- Krakoa/Arakko
- Robert "Bobby" Drake/Iceman
- Romeo (Inhuman)
- Judah Miller (Human)
- Simon Lasker/Pyro II
- Christian Frost/White Bishop
- Dante Ortega (Human)
- Jean-Paul Beaubier/Northstar
- Kyle Jinadu-Beaubier (Human)
- Piotr "Peter" Rasputin/Colossus (only in Ultimate Universe)
- Raul (Human)
- Raymonde Belmonde (Human)
- Kate Pryde/Shadowcat/Red Queen
- Raven Darkholme/Mystique
- Irene Adler/Destiny
- Akihiro/Daken
- Marcus Roston (Human)
- Agent Kim (Human)
- Elizabeth "Betsy" Braddock/Captain Britain
- Xi'an Coy Manh/Karma
- David Alleyne/Prodigy
- Cessily Kincaid/Mercury
- Victor Borkowski/Anole
- Jonas Graymalkin
- Roxanne Washington/Bling!
- Miranda Leevald/Stacy X
- Gaveedra Seven/Shatterstar
- Julio Richter/Rictor
- Myles Alfred/Vivisector
- William Robert Reilly/Phat
- Benjamin Deeds/Morph
- Nathaniel Carver/Hindsight
- Jumbo Carnation
- Darnell Wade/Darkveil/Shade
- Masque (genderfluid)
- Jacob "Jake" Gavin, Jr./Jacqueline "Jackie" Gavin/Courier (genderfluid)
- Sybil Dvorak/Skein
- Solem
- Young Avengers/Champions/Avengers Academy
- Dorrek VIII/Theodore "Teddy" Altman/Hulkling
- William "Billy" Kaplan/Wiccan
- Thomas “Tommy” Shepherd/Speed
- America Chavez/Ms. America
- Ramone Watts/Alloy
- Lisa Halloran
- Elena and Amalia Chavez (parents)
- Noh-Varr/Marvel Boy
- Aikku Jokinen/Enigma/Pod
- Toni Ho/Iron Patriot
- Brandon Sharpe/Striker
- Viv Vision
- Miguel Santos\Living Lightning
- Julie Power/Lightspeed
- Asgardians/Angels
- Runaways
- Villains
- Sinclair Abbott/Spymaster
- Max Dillon/Electro
- Janice Lincoln/Beetle
- Ian Soo/Telekinian
- Sybil Dvorak/Skein
- Deidre Wentworth/Superia
- Heroes
- Peter Quill/Star-Lord
- Hercules
- Hector
- Alana Jobson/Jackpot II
- Curtis Doyle/Freedom Ring
- Val Ventura/Flatman
- Aneka of the Dora Milaje
- Ayo of the Dora Milaje
- Raz Malhotra/Giant-Man IV
- Kyle
- Dennis Dunphy/D-Man
- Phyla-Vell/Martyr
- Heather Douglas/Moondragon
- Jennifer Kale
- Korg
- Ken Shiga/Koi Boi
- Others
Alternate Universes
- Mariko Yashida/Sunfire (only in Earth-2109)
- Mary Jane Watson/Spider-Woman (only in Earth-8545)
- Simon Williams/Wonder Man (only in Earth-763)
- Henry McCoy/Beast (only in Earth-763)
- Jack Storm/Human Torch (only in Earth-93563)
- Reed Richards/Mister Fantastic (only in Earth-93563)
- James Howlett (only in Earth-12025)
- Vanessa Fisk/Madam Kingpin (only in Earth-200111)
- Elektra (only in Earth-200111)
- Jessica Drew/Spider-Woman/Black Widow III (only in Ultimate Universe)
- Arthur Centino/Longshot (only in Ultimate Universe)
- Edith Freiberg/Inertia (only in Supremeverse)
- Jubilation Lee/Jubilee (only in Battlerealm)
- Megan Gwynn/Pixie (only in Battlerealm)
- Leah of Hel (only in Battlerealm)
- Illyana Rasputina/Magik (only in Battlerealm)
- Em Jay Watson (only in Earth-65)
- Glory Grant (only in Earth-65)
Other Media
- Justin Hammer (only in MCU)
- Ellie Phimister/Negasonic Teenage Warhead (only in the Deadpool film franchise)
- Yukio (only in the Deadpool film franchise)
- Lenore "Lenny" Busker
- Clark DeBussy
- Jeryn "Jeri" Hogarth (only in MCU)
- Jose "Joey" Gutierrez
- Hernan "Shades" Alvarez (only in MCU)
- Darius Jones/Comanche (only in MCU)
- Gillian
- Eddy Costa
- Russell Costa
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