The symbiote was, at some pointed, bonded to Spider-Man before he rejected it. It bonded to Eddie Brock shortly afterwards, and the two became Venom.
Much later, Venom was captured by Oscorp so that they may study the symbiote. During the Cosmic Brick crisis, Venom managed to escape and infect numerous Oscorp employees with the symbiote.
When Spider-Man, Hawkeye, and Black Widow followed the Green Goblin to Oscorp, they were sent through a trap door to the lower levels of the building, where Venom's breakout had occurred. Venom stalked the three heroes throughout the entire floor, until he cornered them in a room. Venom fought the three heroes, but was eventually defeated when they exploited his weaknesses to sonic vibrations and fire. His attack, however, gave the Green Goblin just enough time to escape.